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OBJECTIVE@#To determine the relation between serum myostatin with body mass index (BMI) and PaO₂/PaCO₂ in men with chronic obstructive pulmonary disease (COPD).@*METHODS@#A cohort of outpatients with stable COPD was evaluated. We evaluated the myostatin, PaO₂/PaCO₂ and BMI, and the patients were stratified by BMI. The plasma level of myostatin and PaO₂/PaCO₂ was measured by high sensitivity ELISA or blood gas analysis.@*RESULTS@#PaCO₂ and myostatin increased significantly compared with those in the control group (P<0.05), but PaO₂ decreased significantly. There was positive correlation between myostatin and PaCO₂ (P<0.05), and negative correlation between myostatin and BMI/FEV1/pred value/PaO₂ (P<0.05).@*CONCLUSION@#Patients with higher myostatin levels had a lower BMI, lower PaO₂ and higher PaCO₂, with poor pathogenetic condition and prognosis. Myostatin may be a potential treatment target in patients with chronic obstructive pulmonary disease.
Subject(s)
Humans , Male , Blood Gas Analysis , Body Mass Index , Monitoring, Physiologic , Myostatin , Blood , Prognosis , Pulmonary Disease, Chronic Obstructive , BloodABSTRACT
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of Fas ligand (FasL) and fulminant hepatitis B in Han Chinese. Methods HBV infected subjects were enrolled in this case-control study, including 233 cases of inactive HBsAg carrier, 68 patients with fulminant hepatitis B,100 cases of spontaneous hepatitis B clearance, 102 patients with hepatitis B virus (HBV) related cirrhosis and 112 patients with HBV related primary hepatocellular carcinoma. The blood samples and clinical data were collected. FasL-844T/C polymorphisms of enrolled subjects were examined by TaqMan real time fluorescent genotyping polymerase chain reaction (RT-PCR). A adjusted odds ratios (OR)and 95% confidence intervals (CI)were calculated using the Logistic regression model. Results After adjusting the factors of gender and age, binary Logistic regression analyses indicated that the genotype frequencies of FasL-844 CC,CT,TT in inactive HBsAg carriers were 50. 64% ,39. 91% and 9. 44% respectively, and those in cases of fulminant hepatitis B were 79. 41%, 17. 65% and 2. 94%, respectively. The analysis also revealed that FasL-844CC genotype in inactive HBsAg carriers was high risk factor of developing fulminant hepatitis B (OR =4. 729,95%CI:0. 510 - 21. 282,P = 0. 043), while there were no statistic significances in other cases (P>0. 05). Conclusion The inactive HBsAg carriers harboring FasL-844CC may have greater susceptibility to fulminant hepatitis B, which need arouse high attention.
ABSTRACT
Objective:To explore the effect of Glycogen synthase kinase-3?(GSK-3?)on cell proliferation in human lung adenocarcinoma cell line A549 and its potential mechanisms.Methods:MTT assay was employed to evaluate the effects of trichostatin A(TSA)and pretreatment with SB216763(a specific inhibitor of GSK-3?)on the proliferation of A549 cells.Western Blot was employed to analyze the protein levels of GSK-3?and phosphorylated GSK-3?(pGSK-3?).Cell life cycle was examined by flow cytometry analysis.The immunocytochemical method was employed to analyze the protein levels of p27.Results:TSA could inhibit the proliferation of A549 cells in a dose-dependent manner and the cell cycle was arrested in G0/G1 phase in A549 cells treated with TSA.While pre-treatment of A549 cells with SB216763 attenuated TSA induced growth inhibiting and cell cycle arrest.TSA did not alter the levels of GSK-3?but decreased pGSK-3?expression and up-regulated p27 expression.As expected,SB216763 increased the levels of pGSK-3?,consistent with inhibition of GSK-3?activity.Inhibition of GSK-3?activity down regulated p27 protein level.Conclusion:GSK-3?was involved in the inhibition of effect induced by TSA on lung carcinoma cells proliferation process.GSK-3?may exert this effect by stabilization of p27 which may be one of the downstream molecules.